期刊
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
卷 35, 期 6, 页码 480-487出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/jir.2014.0152
关键词
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资金
- National Space Biomedical Research Institute under NASA [MA02501, NCC 9-58]
- DOE-NASA - Office of Science (Biological and Environmental Research), U.S. Department of Energy [DE-SC0001507]
- 2 NASA Postdoctoral Program fellowships from NASA's Space Biology Program
Exposure to ionizing radiation can cause rapid mineral loss and increase bone-resorbing osteoclasts within metabolically active, cancellous bone tissue leading to structural deficits. To better understand mechanisms involved in rapid, radiation-induced bone loss, we determined the influence of total body irradiation on expression of select cytokines known both to stimulate osteoclastogenesis and contribute to inflammatory bone disease. Adult (16 week), male C57BL/6J mice were exposed to either 2Gy gamma rays (Cs-137, 0.8Gy/min) or heavy ions (Fe-56, 600MeV, 0.50-1.1Gy/min); this dose corresponds to either a single fraction of radiotherapy (typical total dose is 10Gy) or accumulates over long-duration interplanetary missions. Serum, marrow, and mineralized tissue were harvested 4h7 days later. Gamma irradiation caused a prompt (2.6-fold within 4h) and persistent (peaking at 4.1-fold within 1 day) rise in the expression of the obligate osteoclastogenic cytokine, receptor activator of nuclear factor kappa-B ligand (Rankl), within marrow cells over controls. Similarly, Rankl expression peaked in marrow cells within 3 days of iron exposure (9.2-fold). Changes in Rankl expression induced by gamma irradiation preceded and overlapped with a rise in expression of other pro-osteoclastic cytokines in marrow (eg, monocyte chemotactic protein-1 increased by 11.9-fold, and tumor necrosis factor-alpha increased by 1.7-fold over controls). The ratio, Rankl/Opg, in marrow increased by 1.8-fold, a net pro-resorption balance. In the marrow, expression of the antioxidant transcription factor, Nfe2l2, strongly correlated with expression levels of Nfatc1, Csf1, Tnf, and Rankl. Radiation exposure increased a serum marker of bone resorption (tartrate-resistant acid phosphatase) and led to cancellous bone loss (16% decrement after 1 week). We conclude that total body irradiation (gamma or heavy-ion) caused temporal elevations in the concentrations of specific genes expressed within marrow and mineralized tissue related to bone resorption, including select cytokines that lead to osteoclastogenesis and elevated resorption; this is likely to account for rapid and progressive deterioration of cancellous microarchitecture following exposure to ionizing radiation.
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