期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 47, 页码 13928-13932出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201506171
关键词
harmonic generation; multimodality imaging; nonlinear optical properties; organized aggregation; porphyrin nanoparticles
资金
- Natural Sciences and Engineering Research Council of Canada
- Canadian Institutes of Health Research
- Terry Fox Research Institute
- Major International (Regional) Joint Research Project from National Science foundation of China
- Canada Foundation for Innovation
- Joey and Toby Tanenbaum/Brazilian Ball Chair in Prostate Cancer Research
- Princess Margaret Cancer Foundation
Nonlinear optical microscopy has become a powerful tool for high-resolution imaging of cellular and subcellular composition, morphology, and interactions because of its high spatial resolution, deep penetration, and low photo-damage to tissue. Developing specific harmonic probes is essential for exploiting nonlinear microscopic imaging for biomedical applications. We report an organized aggregate of porphyrins (OAP) that formed within lipidic nanoparticles showing fingerprint spectroscopic properties, structure-associated second harmonic generation, and superradiant third harmonic generation. The OAP facilitated harmonic microscopic imaging of living cells with significantly enhanced contrast. The structure-dependent switch between harmonic (OAP-intact) and fluorescence (OAP-disrupted) generation enabled real-time multi-modality imaging of the cellular fate of nanoparticles. Robustly produced under various conditions and easily incorporated into pre-formed lipid nanovesicles, OAP provides a biocompatible nanoplatform for harmonic imaging.
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