期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 17, 页码 5069-5073出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201412359
关键词
biomembranes; liposomes; micelles; proteins; surfactants
资金
- Deutsche Akademische Austauschdienst (DAAD) [56041134]
- Deutsche Forschungsgemeinschaft (DFG) through International Research Training Group (IRTG) [1830]
Surfactants carrying fluorocarbon chains hold great promise as gentle alternatives to conventional hydrocarbon-based detergents for the solubilization and handling of integral membrane proteins. However, their inertness towards lipid bilayer membranes has limited the usefulness of fluorinated surfactants in situations where detergent-like activity is required. We demonstrate that fluorination does not necessarily preclude detergency, as exemplified by a fluorinated octyl maltoside derivative termed F6OM. This nonionic compound readily interacts with and completely solubilizes phospholipid vesicles in a manner reminiscent of conventional detergents without, however, compromising membrane order at subsolubilizing concentrations. Owing to this mild and unusual mode of detergency, F6OM outperforms a lipophobic fluorinated surfactant in chaperoning the functional refolding of an integral membrane enzyme by promoting bilayer insertion in the absence of micelles.
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