期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 27, 页码 7795-7798出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201500561
关键词
click chemistry; DNA catenanes; DNA nanotechnology; DNA tiles; stability
资金
- European Commission under the Seventh Framework Programme (FP7), as part of the Marie Curie Initial Training Network, EScoDNA [GA 317110]
- DFG [SFB1032 (A6)]
DNA-based self-assembled nanostructures are widely used to position organic and inorganic objects with nanoscale precision. A particular promising application of DNA structures is their usage as programmable carrier systems for targeted drug delivery. To provide DNA-based templates that are robust against degradation at elevated temperatures, low ion concentrations, adverse pH conditions, and DNases, we built 6-helix DNA tile tubes consisting of 24 oligonucleotides carrying alkyne groups on their 3-ends and azides on their 5-ends. By a mild click reaction, the two ends of selected oligonucleotides were covalently connected to form rings and interlocked DNA single strands, so-called DNA catenanes. Strikingly, the structures stayed topologically intact in pure water and even after precipitation from EtOH. The structures even withstood a temperature of 95 degrees C when all of the 24 strands were chemically interlocked.
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