Early Impairment of Transmural Principal Strains in the Left Ventricular Wall After Short-Term, High-Fat Feeding of Mice Predisposed to Cardiac Steatosis

Background-Myocardial lipid accumulation precedes some cardiomyopathies, but little is known of concurrent effects on ventricular mechanics. We tested the hypothesis that intramyocardial lipid accumulation during a short-term, high-fat diet (HFD) affects 2-dimensional strains in the heart. We examined the hearts of nontransgenic (NTG) mice and of transgenic mice predisposed to elevated triacylglyceride (TAG) storage linked to low-level overexpression of peroxisome proliferator activated receptor (PPAR-alpha). Methods and Results-Myocardial lipid and transmural principal strains E1 and E2 were determined in vivo with H-1 magnetic resonance spectroscopy/imaging before and after 2 weeks of an HFD in both PPAR-alpha and NTG littermate mice. Baseline lipid was elevated in PPAR-alpha compared with NTG mice. An HFD increased mobile lipid by 174% in NTG mice (P<0.05) and by 79% in PPAR-alpha mice (P<0.05). After an HFD, lipid and TAG were higher in PPAR-alpha versus NTG mice by 63% and 81%, respectively. However, TAG in PPAR-alpha mice after an HFD was similar to TAG in PPAR-alpha mice fed a regular diet, suggesting that the magnetic resonance spectroscopy signal from lipid is not exclusive to TAG. Only at the highest lipid contents, achieved in PPAR-alpha mice, were strains affected. Endocardial strain was most compromised, with a negative correlation to lipid (P<0.05). Conclusions-A short-term HFD elevated myocardial lipid measures as determined by magnetic resonance spectroscopy, which became dissociated from TAG content in hearts predisposed to cardiac steatosis. The increased lipid was associated with concurrent, transmural reductions in E1 and E2 strains across the left ventricular wall. Strains were attenuated at the highest levels of lipid accumulation, suggesting a threshold response. Thus, 2-dimensional strains are impaired early and without left ventricular diastolic dysfunction, owing to cardiac steatosis. (Circ Cardiovasc Imaging. 2010; 3: 710-717.)