期刊
CIRCULATION RESEARCH
卷 113, 期 7, 页码 856-862出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCRESAHA.113.302035
关键词
Brachyury protein; embryonic stem cells; epigenomics; Jmjd3 protein; mouse; mesoderm; Wnt signaling pathway
资金
- German Research Foundation [SFB834]
- LOEWE Center for Cell and Gene Therapy Frankfurt [IIIL4-518/17.004[2010]]
Rationale: The developmental role of the H3K27 demethylases Jmjd3, especially its epigenetic regulation at target genes in response to upstream developmental signaling, is unclear. Objective: To determine the role of Jmjd3 during mesoderm and cardiovascular lineage commitment. Methods and Results: Ablation of Jmjd3 in mouse embryonic stem cells does not affect the maintenance of pluripotency and self-renewal but compromised mesoderm and subsequent endothelial and cardiac differentiation. Jmjd3 reduces H3K27me3 marks at the Brachyury promoter and facilitates the recruitment of -catenin, which is critical for Wnt signal-induced mesoderm differentiation. Conclusions: These data demonstrate that Jmjd3 is required for mesoderm differentiation and cardiovascular lineage commitment.
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