期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 39, 页码 11504-11510出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201504249
关键词
bioorthogonal chemistry; fluorescent probes; glycans; glycosylation; sialic acids
资金
- National Science Foundation [DBI-1041078]
- Gordon and Betty Moore Foundation
- National Science Foundation Graduate Research Fellowship
- National Institutes of Health [R37 GM059907]
Vertebrate glycans constitute a large, important, and dynamic set of post-translational modifications that are notoriously difficult to manipulate and image. Although the chemical reporter strategy has been used in conjunction with bioorthogonal chemistry to image the external glycosylation state of live zebrafish and detect tumor-associated glycans in mice, the ability to image glycans systemically within a live organism has remained elusive. Here, we report a method that combines the metabolic incorporation of a cyclooctyne-functionalized sialic acid derivative with a ligation reaction of a fluorogenic tetrazine, allowing for the imaging of sialylated glycoconjugates within live zebrafish embryos.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据