期刊
JOURNAL OF INSECT PHYSIOLOGY
卷 80, 期 -, 页码 71-80出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jinsphys.2015.02.005
关键词
Adoxophyes honmai; Juvenile hormone esterase (JHE); JH epoxide hydrolase (JHEH); Baculovirus; Nucleopolyhedrovirus; qRT-PCR
资金
- Overseas Intensive Short-term Course fellowship of United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology
- Grants-in-Aid for Scientific Research [15J08419] Funding Source: KAKEN
Juvenile hormones (JHs) and ecdysteroids are critical insect developmental hormones. JH esterase (JHE) and JH epoxide hydrolase (JHEH) are JH-selective enzymes that metabolize JH and thus regulate the titer of JH. Baculoviruses are known to alter host endocrine regulation. The nucleopolyhedroviruses, AdhoNPV and AdorNPV, are known to have slow and fast killing activity against Adoxophyes honmai (Lepidoptera: Tortricidae), respectively. Here we found that when penultimate (4th) instar A. honmai are inoculated with AdhoNPV or AdorNPV, the mean survival time is 9.7 and 8.2 days, respectively. The larvae molted once but did not pupate. The AdhoNPV- or AdorNPV-infected larvae did not show a dramatic increase in JHE activity as was found in mock-infected larvae, instead they showed a marked decrease in JHE activity. In contrast, both viral infections had no effect on JHEH activity. In order to further characterize the JHE activity, the JHE-coding sequence of A. honmai (ahjhe) was cloned and confirmed to encode a biologically active JHE. Quantitative real-time PCR analysis of ahjhe expression in 4th and 5th instar A. honmai revealed that AdhoNPV and AdorNPV are able to reduce ahjhe expression levels. (C) 2015 Elsevier Ltd. All rights reserved.
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