期刊
CIRCULATION
卷 124, 期 5, 页码 602-U170出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1161/CIRCULATIONAHA.111.032136
关键词
hypertension; vasodilation; KCNQ potassium channels; gene expression
资金
- Biotechnology and Biological Sciences Research Council [BB/G016321/1]
- British Heart Foundation [PG/09/104, PG/07/127/24235]
- Danish National Research Foundation
- EUFP7 HEALTH grant [240067]
- British Heart Foundation [PG/09/104/28136] Funding Source: researchfish
Background-Voltage-gated potassium (K+) channels encoded by KCNQ genes (Kv7 channels) have been identified in various rodent and human blood vessels as key regulators of vascular tone; however, nothing is known about the functional impact of these channels in vascular disease. We ascertained the effect of 3 structurally different activators of Kv7.2 through Kv7.5 channels (BMS-204352, S-1, and retigabine) on blood vessels from normotensive and hypertensive animals. Methods and Results-Precontracted thoracic aorta and mesenteric artery segments from normotensive rats were relaxed by all 3 Kv7 activators, with potencies of BMS-204352 = S-1>retigabine. We also tested these agents in the coronary circulation using the Langendorff heart preparation. BMS-204352 and S-1 dose dependently increased coronary perfusion at concentrations between 0.1 and 10 mu mol/L, whereas retigabine was effective at 1 to 10 mu mol/L. In addition, S-1 increased K+ currents in isolated mesenteric artery myocytes. The ability of these agents to relax precontracted vessels, increase coronary flow, or augment K+ currents was impaired considerably in tissues isolated from spontaneously hypertensive rats (SHRs). Of the 5 KCNQ genes, only the expression of KCNQ4 was reduced (approximate to 3.7 fold) in SHRs aorta. Kv7.4 protein levels were approximate to 50% lower in aortas and mesenteric arteries from spontaneously hypertensive rats compared with normotensive vessels. A similar attenuated response to S-1 and decreased Kv7.4 were observed in mesenteric arteries from mice made hypertensive by angiotensin II infusion compared with normotensive controls. Conclusions-In 2 different rat and mouse models of hypertension, the functional impact of Kv7 channels was dramatically downregulated. (Circulation. 2011;124:602-611.)
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