期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 144, 期 -, 页码 47-55出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2014.12.012
关键词
Copper(II) compound; Human serum albumin; Cytotoxicity; Cell apoptosis
资金
- Natural Science Foundation of China [31060121, 31460232, 21171043, 21431001]
- Natural Science Foundation of Guangxi [2012GXNSFCB053001, 2013GXNSFGA019010, 2014GXNSFDA118016]
- Technology division of Guilin [20130403-1]
- Guangxi 'Bagui' scholar program
We use Schiff-base salicylaldehyde benzoylhydrazone (HL) as the ligand for copper(II), resulting in the complexes [CuCl(L)]center dot H2O (C1), [CuNO3(L)]center dot H2O (C2) and [CuBr(L)](2) (C3). We characterize the Cu(II) compounds' interactions with human serum albumin (HSA) using fluorescence spectroscopy and molecular docking. These studies revealed that Cu(II) compounds propensity bound to IIA subdomain of HSA possible by hydrophobic interactions and hydrogen bond. Cu(II) compounds produce intracellular reactive oxygen species (ROS) in cancer cells. Complexes of HSA and copper(II) compounds enhance about 2-fold cytotoxicity in cancer cells but do not raise cytotoxicity levels in normal cells in vitro. Compared with C3 alone, HSA-C3 complex promotes HepG2 cell apoptosis and has a stronger capacity to promote cell cycle arrest at the G2/M phase of HepG2. (C) 2014 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据