期刊
JOURNAL OF INORGANIC BIOCHEMISTRY
卷 145, 期 -, 页码 30-40出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jinorgbio.2014.12.020
关键词
Oxidative damage prevention; Sulfur antioxidants; Selenium antioxidants; Antioxidant mechanisms; Copper coordination; Iron coordination
资金
- National Science Foundation grant [CHE 1213912]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1213912] Funding Source: National Science Foundation
Because sulfur and selenium antioxidants can prevent oxidative damage, numerous animal and clinical trials have investigated the ability of these compounds to prevent the oxidative stress that is an underlying cause of cardiovascular disease, Alzheimer's disease, and cancer, among others. One of the most common sources of oxidative damage is metal-generated hydroxyl radical; however, very little research has focused on determining the metal-binding abilities and structural attributes that affect oxidative damage prevention by sulfur and selenium compounds. In this review, we describe our ongoing investigations into sulfur and selenium antioxidant prevention of iron- and copper-mediated oxidative DNA damage. We determined that many sulfur and selenium compounds inhibit Cu-I-mediated DNA damage and that DNA damage prevention varies dramatically when Fe-II is used in place of Cu-I to generate hydroxyl radical. Oxidation potentials of the sulfur or selenium compounds do not correlate with their ability to prevent DNA damage, highlighting the importance of metal coordination rather than reactive oxygen species scavenging as an antioxidant mechanism. Additional gel electrophoresis, mass spectrometry, and UV-visible studies confirmed sulfur and selenium antioxidant binding to Cu-I and Fe-II. Ultimately, our studies established that both the hydroxyl-radical-generating metal ion and the chemical environment of the sulfur or selenium significantly affect DNA damage prevention and that metal coordination is an essential mechanism for these antioxidants. (C) 2015 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据