期刊
ACS NANO
卷 9, 期 7, 页码 6696-6705出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b01043
关键词
agglomeration; clusters; protein corona; time-dependent Smoluchowiski agglomeration model; protein-nanoparticle mixtures; dispersion stability; electrosteric stabilization; particle stability
类别
资金
- INM
Citrate-stabilized gold nanoparticles (AuNP) agglomerate in the presence of hemoglobin (Fib) at acidic pH. The extent of agglomeration strongly depends on the concentration ratio [Hb]/[AuNP]. Negligible agglomeration occurs at very low and very high [Hb]/[AuNP]. Full agglomeration and precipitation occur at [Hb]/[AuNP] corresponding to an Hb monolayer on the AuNP. Ratios above and below this value lead to the formation of an unexpected phase: stable, microscopic AuNP Hb agglomerates. We investigated the kinetics of agglomeration with dynamic light scattering and the adsorption kinetics of Hb on planar gold with surface-acoustic wave-phase measurements. Comparing agglomeration and adsorption kinetics leads to an explanation of the complex behavior of this nanoparticle-protein mixture. Agglomeration is initiated either when lib bridges AuNP or when the electrostatic repulsion between AuNP is neutralized by Hb. It is terminated when Hb has been depleted or when Hb forms multilayers on the agglomerates that stabilize microscopic clusters indefinitely.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据