期刊
CIENCIAS MARINAS
卷 35, 期 2, 页码 153-167出版社
INST INVESTIGACIONES OCEANOLOGICAS, U A B C
DOI: 10.7773/cm.v35i2.1525
关键词
16S rDNA RFLP; marine bacteria; South China Sea; Straits of Malacca; ZoBell 2216E
资金
- Southeast Asia Regional Committee for START (SARCS) [94/01/CW, 95/01/CW]
- University of Malaya [FP013/2004B]
- National Oceanography Directorate of Malaysia [04-01-03-SF0194]
In this Study we isolated and characterized some culturable bacteria from tropical coastal waters of Peninsular Malaysia. We obtained between 0.23 and 1.85 x 10(3) cfu mL(-1) in the Zobell 2216E medium, and cultured 0.04% to 0.12% of total bacterial counts. Different bacterial strains were then selected by 16S rDNA RFLP using four restriction enzymes (DdeI, HhaI, RsaI, and Sau3AI), of which HhaI gave the most RFLP patterns. A total of 54 unique strains were obtained and these were identified by their 16S rDNA gene sequence. These bacterial strains could be divided into five classes: 38 strains of gamma-Proteobacteria (61.1%); 3 strains of alpha-Proteobacteria (5.5%); 2 strains of the Cytophaga-Flavobacterium-Bacteroides group (3.7%); 3 strains of high GC, Gram-positive bacteria (5.5%); and 13 strains of low GC, Gram-positive bacteria (24.1%). These isolates have good potential for further biotechnological studies since about 56% of the isolates exhibited amylase activity, whereas 36% and 18% of the isolates had protease and lipase, respectively. Most (>70%) of the isolates also produced poly-beta-hydroxybutyrate.
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