期刊
JOURNAL OF INFECTIOUS DISEASES
卷 212, 期 6, 页码 999-1008出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiv142
关键词
amplicon sequencing; deep sequencing; genetic diversity; hypnozoite; malaria; microsatellite; multiplicity of infection; Plasmodium vivax; pvmsp1; relapse
资金
- National Institutes of Health [K08 AI110651, R01 AI089819, R01 AI099473-03]
- US Army Medical Materiel Development Activity, Fort Detrick, MD
- American Society of Tropical Medicine and Hygiene (ASTMH)/Burroughs Wellcome Postdoctoral Fellowship in Tropical Infectious Disease
Plasmodium vivax infections often recur due to relapse of hypnozoites from the liver. In malaria-endemic areas, tools to distinguish relapse from reinfection are needed. We applied amplicon deep sequencing to P. vivax isolates from 78 Cambodian volunteers, nearly one-third of whom suffered recurrence at a median of 68 days. Deep sequencing at a highly variable region of the P. vivax merozoite surface protein 1 gene revealed impressive diversity-generating 67 unique haplotypes and detecting on average 3.6 cocirculating parasite clones within individuals, compared to 2.1 clones detected by a combination of 3 microsatellite markers. This diversity enabled a scheme to classify over half of recurrences as probable relapses based on the low probability of reinfection by multiple recurring variants. In areas of high P. vivax diversity, targeted deep sequencing can help detect genetic signatures of relapse, key to evaluating antivivax interventions and achieving a better understanding of relapse-reinfection epidemiology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据