期刊
JOURNAL OF INFECTIOUS DISEASES
卷 212, 期 5, 页码 830-838出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiv128
关键词
cytokines; human T-cells; memory response; proliferation; S. aureus
资金
- Deutsche Forschungsgemeinschaft [SFB-TR34 and GRK840]
- Federal Ministry of Education and Research (HICARE and Infection Genomics)
- Alfried Krupp Wissenschaftskolleg
Introduction. Though Staphylococcus aureus is a major pathogen, vaccine trials have failed. In contrast, class-switched antibodies specific to S. aureus are common, implying immune memory formation and suggesting a large pool of S. aureus-reactive helper T-cells. Objective. To elucidate the cellular arm of S. aureus-specific immune memory, the T-cell response in humans was characterized. Methods. The proliferative response of human peripheral blood mononuclear cells (PBMCs) to S. aureus antigens and the frequency of S. aureus-specific T-cells were quantified by H-3-thymidine incorporation; cytokine release was measured by flow cytometry. Results. Staphylococcus aureus particles and extracellular proteins elicited pronounced proliferation in PBMCs of healthy adults. This reflected a memory response with high frequencies of T-cells being activated by single S. aureus antigens. The whole S. aureus-specific T-cell pool was estimated to comprise 3.6% of T-cells with 35-fold differences between individuals (range, 0.2%-5.7%). When exposed to S. aureus antigens, the T-cells released predominantly but not solely T helper (Th) 1/Th17 cytokines. Conclusions. The large number of S. aureus antigen-reactive memory T-lymphocytes is likely to influence the course of S. aureus infection. To enable rational vaccine design, the naturally acquired human T-cell memory needs to be explored at high priority.
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