期刊
CHROMOSOME RESEARCH
卷 22, 期 3, 页码 267-276出版社
SPRINGER
DOI: 10.1007/s10577-013-9400-x
关键词
Sister chromatid cohesion; CDK11; Centromere; Mitosis; Mammalian cells
资金
- Ligue Contre le Cancer/Region Bretagne fellowship
- French National Research Agency (ANR, project EpiCentr)
- Region Bretagne (SAD grant)
- Canceropole Grand-Ouest
- Ligue Contre le Cancer (Comite Grand Ouest)
- Fondation ARC pour la Recherche sur le Cancer
The cyclin-dependent kinase CDK11(p58) is specifically expressed at G2/M phase. CDK11(p58) depletion leads to different cell cycle defects such as mitotic arrest, failure in centriole duplication and centrosome maturation, and premature sister chromatid separation. We report that upon CDK11 depletion, loss of sister chromatid cohesion occurs during mitosis but not during G2 phase. CDK11(p58) depletion prevents Bub1 and Shugoshin 1 recruitment but has no effect on the dimethylation of histone H3 lysine 4 at centromeres. We also report that a construct expressing a kinase dead version of CDK11(p58) fails to prevent CDK11 depletion-induced sister chromatid separation, showing that CDK11(p58) kinase activity is required for protection of sister chromatid cohesion at centromeres during mitosis. Thus, CDK11(p58) kinase activity appears to be involved in early events in the establishment of the centromere protection machinery.
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