期刊
CHROMOSOME RESEARCH
卷 19, 期 1, 页码 83-98出版社
SPRINGER
DOI: 10.1007/s10577-010-9172-5
关键词
binding models; FRAP; high mobility; transient chromatin binding; diffusion; transcription; DNA repair
Fluorescent protein labelling, as well as impressive progress in live cell imaging have revolutionised the view on how essential nuclear functions like gene transcription regulation and DNA repair are organised. Here, we address questions like how DNA-interacting molecules find and bind their target sequences in the vast amount of DNA. In addition, we discuss methods that have been developed for quantitative analysis of data from fluorescence recovery after photobleaching experiments (FRAP).
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