期刊
CHROMATOGRAPHIA
卷 76, 期 13-14, 页码 821-829出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-013-2479-6
关键词
Microchip electrophoresis; Light-emitting diode; Fluorescence detection; Sulfonamides
资金
- National Natural Science Foundation of China [20727006]
- Guangdong Provincial Science and Technology Project [2008A030102009]
In this paper, we describe a compact and low-cost light-emitting diode-induced fluorescence (LED-IF) detection coupled to microchip electrophoresis for the determination of sulfonamides in pharmaceutical formulations and rabbit plasma. Three fluorescein isothiocyanate-labeled sulfonamides in rabbit plasma were separated in the running buffer of 40 mM phosphate buffer (pH 7.0) at the separation voltage of 2.0 kV, and detected by LED-IF detector in which the high-power blue LED was driven at the constant current of 150 mA and the emitted fluorescence over 510 nm was collected by a planar photodiode. The linear concentration ranged from 2.0 to 125.0 mu g mL(-1), both for sulfadiazine and sulfamethazine with the correlation coefficients (r (2)) of 0.995 and 0.997, respectively, and from 2.0 to 100.0 mu g mL(-1) with the correlation coefficients (r (2)) of 0.997 for sulfaguanidine. The limits of detection for the three sulfonamides were 0.36-0.50 mu g mL(-1) (S/N = 3). Intra-day and inter-day precision of migration time and peak area for the determination of sulfonamides were < 4.5 %. This method has been successfully applied to the analysis of sulfonamides in pharmaceuticals, and could be used to study the pharmacokinetics of sulfonamides in rabbit.
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