期刊
CHROMATOGRAPHIA
卷 73, 期 -, 页码 S137-S142出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-010-1878-1
关键词
Column liquid chromatography; UV-detection; Mass spectrometry; Isothiocyanates; Solubility; Mercaptoethanol
Numerous isothiocyanates (ITCs) are poorly soluble in water which causes their precipitation in aqueous mobile phases used in reversed phase liquid chromatography (RP-LC), thus impacting the accuracy of the quantification. By comparing the amounts of ITCs injected and released from the column, losses could be estimated at 5-32% depending on polarities and concentrations. Results could be dramatically improved in terms of separation and quantification using RP-LC with a mercaptoethanol precolumn derivatization aimed at avoiding ITCs precipitation. The cancer chemoprotective allyl-ITC and sulforaphane were found in cabbage extracts at 1.2 and 2.7 mu g g(-1) fresh weight, respectively.
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