Analysis of Volatile Compounds in the Pericarp of Zanthoxylum bungeanum Maxim. by Ultrasonic Nebulization Extraction Coupled with Headspace Single-Drop Microextraction and GC-MS

Ultrasonic nebulization extraction (UNE) combined with headspace single-drop microextraction (HS-SDME) and gas chromatography-mass spectrometry (GC-MS) have been used for rapid analysis of volatile components of the pericarp of Zanthoxylum bungeanum Maxim. Effective extraction was achieved by suspending 2 mu L n-heptadecane from the tip of a microsyringe, extracting for 15 min, and enriching for 25 min. The method combines the advantages of UNE and HS-SDME. Thirty-four compounds were identified in the pericarp of Zanthoxylum bungeanum Maxim. Compared with stirring extraction (SE)-HS-SDME and UNE, UNE-HS-SDME was resulted in higher extraction yield, enrichment efficiency, and sensitivity. The results indicate that UNE-HS-SDME is a feasible alternative method for analysis of essential oils from spices.