期刊
CHROMATOGRAPHIA
卷 72, 期 9-10, 页码 969-973出版社
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-010-1745-0
关键词
Hydrophilic interaction liquid chromatography; LC-ESI-MS-MS; Feed samples; Sanguinarine and chelerythrine
A simple, rapid and sensitive LC-MS method utilizing electrospray mass spectrometry (ESI-MS-MS) has been developed to determine sanguinarine and chelerythrine in feed samples. Chromatographic separation was performed on a hydrophilic interaction liquid chromatography column using acetonitrile-25 mM ammonium formate buffer pH 3.2 (80 + 20 v/v) as mobile phase. Under these LC conditions, the retention times of chelerythrine and sanguinarine were 3.9 and 4.2 min, respectively. The observed fragment ions were 317, 304 and 274 m/z for sanguinarine and 332, 304 and 290 m/z for chelerythrine. The lowest concentration of standard solution injected on column and detected by LC-MS-MS was 0.15 and 0.10 mu g L(-1) for sanguinarine and chelerythrine. The limits of detection and of quantification values in feed samples were 7.5 and 25 mu g kg(-1) for sanguinarine, 5 and 15 mu g kg(-1) for chelerythrine. The recovery of the method as well as the intra-day and inter-day precision were satisfactory.
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