Determination of Sudan Dyes and Para Red in Duck Muscle and Egg by UPLC

A simple, sensitive and reliable UPLC method was developed and validated for the simultaneous determination of six Sudan dyes (Sudan Red G, Sudan I, II, III, Sudan Red 7B, Sudan IV) and Para Red in duck muscle and egg samples. Samples were extracted with acetonitrile, then the extract was dried under rotary evaporation and dissolved in acetonitrile/0.1% formic acid (85:15, v/v). Chromatographic separation was achieved on an Acquity UPLC BEH C18 column under gradient conditions. Samples were detected using a diode array detector set to 480 and 500 nm. The method was validated with respect to linearity, accuracy, precision, and specificity.