期刊
CHROMATOGRAPHIA
卷 68, 期 11-12, 页码 885-892出版社
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-008-0859-0
关键词
Counter-current chromatography; Flavonoids; Oroxylum indicum
资金
- National Natural Science Foundation of China [NSFC-30572311]
- New Century Excellent Talent Foundation of China [NCET-05-07889]
A high-speed counter-current chromatography system (HPCCC) capable of rapid processing has been employed to separate seven flavonoids from a methanolic extract of the leaves of Oroxylum indicum by a one-step isocratic elution using a chloroform-methanol-water (9.5:10:5) two-phase system. LC, MS and NMR have identified the components from the extract as chrysin, baicalein, baicalein-7-O-glucoside, baicalein-7-O-diglucoside, chrysin-7-O-glucuronide, baicalein-7-O-glucuronide, and a chrysin-diglucoside. Baicalein-7-O-glucuronide and chrysin-7-O-glucuronide have been separated from this plant by HSCCC for the first time. The present study also reports a new chrysin-diglucoside from the leaf extract. The results demonstrate that HSCCC is a powerful separation tool and can contribute to identifying and quantifying plant ingredients.
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