期刊
CHROMATOGRAPHIA
卷 68, 期 7-8, 页码 623-627出版社
SPRINGER HEIDELBERG
DOI: 10.1365/s10337-008-0729-9
关键词
column liquid chromatography-tandem mass spectrometry; nicotine metabolites in human urine
资金
- National Nature Science Foundation of China [20705040]
- Scientific Foundation of State Tobacco Monopoly Administration of China
A method based on liquid chromatography tandem mass spectrometry was developed for the direct determination of nicotine, cotinine, trans-3'-hydroxycotinine, their corresponding glucuronide conjugates as well as nornicotine, norcotinine, cotinine-N-oxide and nicotine-N'-oxide in the urine of smokers. The assay only involves centrifugation and filtration of diluted urine. The analysis was performed on a C18 reversed-phase column using a gradient of 10 mM ammonium acetate, pH 6.8, and methanol as mobile phase at a flow rate of 1 mL min(-1). Nicotine-methyl-d(3), Cotinine-methyl-d(3) and trans-3'-hydroxycotinine-methyl-d(3) were used as internal standards. Precisions (RSD) for all the analytes at three levels were between 2.1 and 17.0%. Recoveries for nicotine and nine nicotine metabolites ranged from 78.4 to 115.6%. The described method was suitable for determining the nicotine dose in large-scale human biomonitoring studies.
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