LC with novel electrochemical detection for analysis of monoamine neurotransmitters in rat brain after administration of (R)-salsolinol and (R)-N-methylsalsolinol

A novel electrochemical detector with an acetylcholine film has been developed for HPLC. The chemically modified electrode is an efficient electrocatalyst of (R)-salsolinol ((R)-Sal), (R)-N-methylsalsolinol ((R)-NMSal), and monoamine neurotransmitters, enabling highly sensitive detection. The electrode is also stable and long-lived. Combined with microdialysis sampling, HPLC with the novel detector enabled successful study of changes in the concentrations of monoamine neurotransmitters in rat brain after the injection of (R)-salsolinol and (R)-N-methylsalsolinol.