期刊
JOURNAL OF INFECTIOUS DISEASES
卷 213, 期 6, 页码 975-984出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/infdis/jiv536
关键词
dengue virus; neutralizing antibodies; envelope protein; fitness; evolution; genotype; lineage; Vietnam; Cambodia
资金
- Bill and Melinda Gates Foundation
- Instituto Carlos Slim de la Salud (FIRST Program)
- Pediatric Dengue Vaccine Initiative
- National Institutes of Health (NIH) [R01 AI099631]
- Gates Cambridge Scholarship
- NIH Oxford-Cambridge Scholars Program
The 4 dengue virus serotypes (DENV-1-4) cause the most prevalent mosquito-borne viral disease of humans worldwide. DENV-2 Asian 1 (A1) genotype viruses replaced the Asian-American (AA) genotype in Vietnam and Cambodia, after which A1 viruses containing Q or M at envelope (E) residue 160 became more prevalent than those with residue 160K in both countries (2008-2011). We investigated whether these substitutions conferred a fitness advantage by measuring neutralizing antibody titer against reporter virus particles (RVPs) representing AA, A1-160K, A1-160Q, and A1-160M, using patient sera from Vietnam and a well-characterized Nicaraguan cohort. Surprisingly, we found that A1-160Q and A1-160M RVPs were better neutralized by heterologous antisera than A1-160K. Despite this, Vietnamese patients infected with A1-160Q or A1-160M viruses had higher viremia levels than those infected with A1-160K. We thus found that independent lineages in Vietnam and Cambodia acquired a substitution in E that significantly increased polyclonal neutralization but nonetheless were successful in disseminating and infecting human hosts.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据