期刊
CHIRALITY
卷 25, 期 7, 页码 415-421出版社
WILEY
DOI: 10.1002/chir.22181
关键词
metal-affinity magnetic nanoparticles; his-tagged protein purification; benzoylformate decarboxylase (BFD); carboligation reactions
资金
- Scientific and Technological Research Council of Turkey [109T497]
In this study, magnetic nanoparticles (Fe3O4, magnetite) with immobilized metal affinity ligands (MSS) were prepared and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), fourier transform infrared spectroscopy (FTIR), and vibrating-sample magnetometer (VSM) methods for purification and immobilization of the histidine-tagged recombinant benzoylformate decarboxylase (BFD). The MSS support was shown to be eligible for selective binding of HIS-tagged BFD by SDS-page analysis. Loading capacity of the MSS support was determined as 43.6 +/- 1.1mg/g. The regeneration ability for protein binding was also studied. An immobilized BFD was tested to catalyze benzoin condensation and representative cross acyloin reaction. Conversion and enantiomeric excess values were comparable with that of free enzyme catalyzed reactions. (C) 2013 Wiley Periodicals, Inc.
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