期刊
JOURNAL OF INFECTION
卷 71, 期 5, 页码 553-560出版社
W B SAUNDERS CO LTD
DOI: 10.1016/j.jinf.2015.07.010
关键词
Hypervirulent K. pneumoniae; Carbapenem-resistance; Clinical features; Molecular characteristics; Virulence
资金
- National Natural Science Foundation [81572036]
- Beijing Natural Science Foundation [5122041]
- Beijing City Board of Education Science and Technology Key Project [KZ201210025025]
- Specialized Research Fund for the Doctoral Program of Higher Education [20110001110043]
- Key Projects in the National Science & Technology Pillar Program [2012EP001002]
Objectives: Hypervirulent Klebsiella pneumoniae (hvKP) infections occur worldwide, but carbapenem-resistant hvKP strain has rarely been observed. Methods: A retrospective study was conducted in 28 cases of carbapenem-resistant K. pneumoniae (CRKP) infections from 9 cities in China. Clinical data were collected from medical records. All the isolates were characterized by antimicrobial susceptibility testing, string test, multilocus sequence typing, and capsular genotyping. All the hypermucoviscous CRKP strains were analyzed by virulence gene profiles, serum killing assay, and mouse lethality assay. Results: Of 28 CRKP isolates, five were positive for string test. Importantly, one of the hypermucoviscous strains isolated from blood sample was identified as hvKP. The hypervirulent CRKP strain showed highly resistant to carbapenems (MIC > 32 mu g/mL), decreased expression of ompK35/36, and ESBLs production. Significantly increased resistance to serum killing and mice mortality were found in the hypervirulent CRKP strain compared to the other CRKPs. Capsular polysaccharide synthesis genotyping revealed that the hypervirulent strain belongs to K2 serotype, while others belong to K-nontypable serotype. The K2 hypervirulent CRKP strain carried rmpA, aerobactin, entB, and mrkD genes. Conclusions: The newly emerged hypervirulent carbapenem-resistant K. pneumoniae might cause a serious threat to public health, suggesting an urgent need to enhance clinical awareness and epidemiologic surveillance. (C)( 2015 The British Infection Association. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据