期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 42, 期 11, 页码 1463-1472出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-015-1674-x
关键词
Fatty alcohols; Metabolic engineering; Fatty acyl-CoA reductase; Rhodosporidium toruloides
资金
- Industrial Technology Development Center (CDTI)
- IDEA Agency
- European Regional Development Fund (ERDF)
We have engineered Rhodosporidium toruloides to produce fatty alcohols by expressing a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8. Production of fatty alcohols in flasks was achieved in different fermentation media at titers ranging from 0.2 to 2 g/L. In many of the conditions tested, more than 80 % of fatty alcohols were secreted into the cultivation broth. Through fed-batch fermentation in 7 L bioreactors, over 8 g/L of C-16-C-18 fatty alcohols were produced using sucrose as the substrate. This is the highest titer ever reported on microbial production of fatty alcohols to date.
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