期刊
JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
卷 42, 期 8, 页码 1117-1128出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10295-015-1631-8
关键词
S. cerevisiae spore; D-Xylose isomerase; D-Psicose 3-epimerase; Enzyme immobilization; D-Psicose synthesis
资金
- National Natural Science Foundation of China [21302069]
- China Postdoctoral Science Foundation [2014M551500]
- Key Project of Chinese Ministry of Education [313027]
- Fundamental Research Funds for the Central Universities [JUSRP1003]
- 111 Project [111-2-06]
- Agriculture Science Technology Achievement Transformation Fund [2014GB2A000268]
Saccharomyces cerevisiae spores are dormant cells, which can tolerate various types of environmental stress. In our previous work, we successfully developed biological and chemical methods for enzyme immobilization based on the structures of S. cerevisiae spore wall. In this study, we employed biological and chemical approaches for the immobilization of d-xylose isomerase (XI) from Thermus thermophilus and d-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens with yeast spores, respectively. The enzymatic properties of both immobilized XI and DPEase were characterized and the immobilized enzymes exhibit higher thermostability, broader pH tolerance, and good repeatability compared with free enzymes. Furthermore, we established a two-step approach for the bioconversion of d-glucose to d-psicose using immobilized enzymes. To improve the conversion yield, a multi-pot strategy was adopted for d-psicose production by repeating the two-step process continually. As a result, the yield of d-psicose was obviously improved and the highest yield reached about 12.0 %.
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