Investigation the activity and stability of lysozyme on presence of magnetic nanoparticles

In this work the influence of Fe2O3 magnetic nanoparticle on lysozyme activity and thermal stability were investigated. The effect of nano-Fe2O3 on lysozyme activity was studied by UV-vis spectrophotometry at 35 degrees C in pH = 7.25 using sodium phosphate as a buffer. Measurements were carried out using 6 x 10(-8) M (0.1 mg/ml) of lysozyme and a range of nano-Fe2O3 concentrations between 0 and 0.045 mg/ml. It was found that by increasing the concentration of nano-Fe2O3, the rate of Micrococcus lysodeikticus lyses (lysozyme activity) will be increased. On the other hand, the thermal stability of lysozyme was investigated in the presence of nano-Fe2O3 over the temperature range (from 293 K to 363 K). Results indicate that by increasing of nano-Fe2O3, the thermal stability of lysozyme will be increased. (C) 2014 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.