期刊
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
卷 21, 期 -, 页码 862-867出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jiec.2014.04.024
关键词
Lysozyme; Magnetic nanoparticle; Spectrophotometry; Protein
资金
- University of Shahrekord
- council of University of Kashan
In this work the influence of Fe2O3 magnetic nanoparticle on lysozyme activity and thermal stability were investigated. The effect of nano-Fe2O3 on lysozyme activity was studied by UV-vis spectrophotometry at 35 degrees C in pH = 7.25 using sodium phosphate as a buffer. Measurements were carried out using 6 x 10(-8) M (0.1 mg/ml) of lysozyme and a range of nano-Fe2O3 concentrations between 0 and 0.045 mg/ml. It was found that by increasing the concentration of nano-Fe2O3, the rate of Micrococcus lysodeikticus lyses (lysozyme activity) will be increased. On the other hand, the thermal stability of lysozyme was investigated in the presence of nano-Fe2O3 over the temperature range (from 293 K to 363 K). Results indicate that by increasing of nano-Fe2O3, the thermal stability of lysozyme will be increased. (C) 2014 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.
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