期刊
JOURNAL OF IMMUNOLOGY
卷 194, 期 4, 页码 1763-1775出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.1401624
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资金
- National Institutes of Health [R01 EY14362, R01 GM36387]
- Visual Sciences Research Center Core Grant [P30 EY11373]
- Cytometry and Imaging Microscopy Core Facility of the Comprehensive Cancer Center of Case Western Reserve University
- University Hospitals of Cleveland Core Grant [P30 CA43703]
- Research to Prevent Blindness Foundation
- Ohio Lions Eye Research Foundation
- Alcon Research Institute award
Although neutrophils are the most abundant cells in acute infection and inflammation, relatively little attention has been paid to their role in inflammasome formation and IL-1b processing. In the present study, we investigated the mechanism by which neutrophils process IL-1 beta in response to Streptococcus pneumoniae. Using a murine model of S. pneumoniae corneal infection, we demonstrated a requirement for IL-1 beta in bacterial clearance, and we showed that Nod-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase activation and recruitment domain (ASC), and caspase-1 are essential for IL-1 beta production and bacterial killing in the cornea. Neutrophils in infected corneas had multiple specks with enzymatically active caspase-1 (YVAD-FLICA 660), and bone marrow neutrophils stimulated with heat-killed S. pneumoniae (signal 1) and pneumolysin (signal 2) exhibited multiple specks when stained for NLRP3, ASC, or Caspase-1. High-molecular mass ASC complexes were also detected, consistent with oligomer formation. Pneumolysin induced K+ efflux in neutrophils, and blocking K+ efflux inhibited caspase-1 activation and IL- 1 beta processing; however, neutrophils did not undergo pyroptosis, indicating that K+ efflux and IL-1 beta processing is not a consequence of cell death. There was also no role for lysosomal destabilization or neutrophil elastase in pneumolysin-mediated IL-1 beta processing in neutrophils. Taken together, these findings demonstrate an essential role for neutrophil-derived IL-1 beta in S. pneumoniae infection, and they elucidate the role of the NLRP3 inflammasome in cleavage and secretion of IL-1 beta in neutrophils. Given the ubiquitous presence of neutrophils in acute bacterial and fungal infections, these findings will have implications for other microbial diseases.
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