期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 16, 页码 4904-4908出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201412049
关键词
biocatalysis; cell encapsulation; interfaces; nanostructures; phase transfer
资金
- National Basic Research Program of China [2012CB720602, 2011CB936004]
- National Natural Science Foundation of China [21210002, 91213302, 21431007, 21202158, 91413111]
A powerful strategy for long-term and diffusional-resistance-minimized whole-cell biocatalysis in biphasic systems is reported where individually encapsulated bacteria are employed as robust and recyclable Pickering interfacial biocatalysts. By individually immobilizing bacterial cells and optimizing the hydrophobic/hydrophilic balance of the encapsulating magnetic mineral shells, the encased bacteria became interfacially active and locate at the Pickering emulsion interfaces, leading to dramatically enhanced bioconversion performances by minimizing internal and external diffusional resistances. Moreover, insitu product separation and biocatalyst recovery was readily achieved using a remote magnetic field. Importantly, the mineral shell effectively protected the entire cell from long-term organic-solvent stress, as shown by the reusability of the biocatalysts for up to 30cycles, while retaining high stereoselective catalytic activities, cell viabilities, and proliferative abilities.
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