Facile Fabrication of Polymeric Ionic Liquid Grafted Porous Polymer Monolith for Mixed-Mode High Performance Liquid Chromatography

A novel polymeric ionic liquid grafted porous polymer monolith has been facilely fabricated for mixed-mode chromatography. The column is prepared from poly (glycidyl methacrylate-co-ethylene dimethacrylate) monolith through hydrolyzation of the epoxy moieties into hydroxyl groups, followed by grafting from polymerization of ionic liquid of 1-vinyl-3-butylimidazolium chloride. Successful modification is characterized by scanning electron microscope, infrared spectroscopy, elemental analysis and mercury intrusion porosimetry. The HPLC performance of developed column is evaluated by separating acidic vitamin B analytes, neutral steroids and basic aromatic amines in mixed-mode chromatography on a single column, respectively. The ionic liquid affords the monolith with both enhanced separation ability and improved column efficiency.