期刊
CHINESE JOURNAL OF CHEMISTRY
卷 29, 期 4, 页码 835-839出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cjoc.201190166
关键词
3-aminophenylboronic acid monohydrate; magnetic mesoporous silica; selective enrichment; glycopeptides; mass spectrometry
资金
- National Natural Science Foundation of China [20971041, 20803020]
- Hunan Provincial Education Department [B30907]
- Doctoral Science Foundation of Hunan University of Science and Technology [E50839]
In the work, aminophenylboronic acid (APB)-functionalized magnetic mesoporous silica, which holds the attractive features of high magnetic responsivity and large surface area, was developed to enrich glycopeptides. At first, magnetic mesoporous silica nanocomposites were prepared. And then, the nanocomposites were functioned with glycidoxypropyltrimethoxysilane (GLYMO) for boronic acid immobilization. Due to that the boronic acid group on the surface of magnetic mesoporous silica nanocomposites can form tight yet reversible covalent bond with glycopeptides containing cis-1,2-diols groups, the magnetic mesoporous silica nanocomposites were successfully applied to selective enrichment of glycopeptides. APB functionalized magnetic mesoporous silica was also demonstrated to have high selectivity for the glycopeptides in the presence of a 10-fold excess bovine serum albumin (BSA) over horseradish peroxidase (HRP) in the tryptic digest. We also find that magnetic mesoporous silica has better sensitivity in HRP digest compared with that of commercial aminophenylboronic acid-functionalized magnetic nanoparticles beads. The limit of detection for glycopeptides from glycoprotein HRP is about 0.01 ng/mu L.
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