Extraction and Determination of Quercetin and Myricetin from Chamaecyparis obtusa by Ionic Liquids-based Monolithic Cartridge

A short ionic liquids (ILs)-based monolithic cartridge was prepared and used as the selective extraction sorbent. After the material was evaluated by field emission-scanning electron microscopy (FE-SEM), a new approach for the extraction and determination of quercetin and myricetin from Chamaecyparis obtusa (C. obtusa) by using ILs-based, monolithic cartridge system was developed. Chromatographic analysis was conducted on a C-18 column with UV detection at 372 nm, an eluting solution consisting of acetonitrile-water (25/75, V/V) as the mobile phase, and a flow rate of 0.7 mL.min(-1). A good linear relationship was demonstrated when the concentrations of quercetin and myricetin were in the range of 0.5-100.0 mu g.mL(-1). The recoveries ranged from 101.6% to 104.6% and the inter- and intra-day relative standard deviations (RSD) were less than 5.0%. This method effectively removed the impurities and avoided tedious pretreatment. It provided a fast, economic and effective method for assaying trace drugs from natural plants.