期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 5, 页码 1662-1665出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201507097
关键词
aspergillus; biosynthesis; genetic dereplication; natural products; NRPS
资金
- H. L. Snyder Medical Foundation
- Irving S. Johnson Fund of the University of Kansas Endowment
- National Institute of General Medical Sciences [P01-GM084077]
To reduce the secondary metabolite background in Aspergillus nidulans and minimize the rediscovery of compounds and pathway intermediates, we created a genetic dereplication strain in which we deleted eight of the most highly expressed secondary metabolite gene clusters (more than 244,000 base pairs deleted in total). This strain allowed us to discover a novel compound that we designate aspercryptin and to propose a biosynthetic pathway for the compound. Interestingly, aspercryptin is formed from compounds produced by two separate gene clusters, one of which makes the well-known product cichorine. This raises the exciting possibility that fungi use differential regulation of expression of secondary metabolite gene clusters to increase the diversity of metabolites they produce.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据