期刊
CHINESE JOURNAL OF CHEMICAL ENGINEERING
卷 19, 期 1, 页码 128-134出版社
CHEMICAL INDUSTRY PRESS CO LTD
DOI: 10.1016/S1004-9541(09)60188-9
关键词
Bacillus subtilis; growth-promoting; diseases control; antifungal chitinase; purification; Rhizoctonia solani
资金
- National Natural Science Foundation of China [20776017]
- Xinjiang Uygur Autonomous Region High-tech Research and Development Project [20081108]
- Fok Ying Tung Education Foundation [101071]
- Xinjiang Bingtuan Key Science and Technology Industry [2008GG24]
The seed germination and tomato seedling tests showed that Bacillus subtilis SL-13 could promote the sprouting and seedling growth of tomato. The fresh and dry weight of tomato seedlings increased 42.86% and 18.75%, respectively. The control efficacies of the SL-13 to tomato Rhizoctonia rot were 20.65% and 35.23% in the greenhouse and field, respectively. The growth of the plant-pathogenic fungus Rhizoctonia solani was considerably inhibited in the presence of the strain SL-13 culture supernatant. The main antifungal protein was detected to be chitinase through vitro assay. The chitinase was purified with DEAE-Sepharose fast flow ion exchange column chromatography and Sephadex G-75 gel filtration for further characterization. The optimal pH and temperature for the chitinase activity were 7.0 and 50 degrees C, respectively. It was demonstrated that the enzyme was stable at pH 5-9 and 40-60 degrees C. 70% of the enzyme activity was retained when incubated at 121 degrees C and 0.11 MPa for 20 min, and the enzyme was not sensitive to protease K and ultraviolet radiation. Thus it is suitable for effective biological control in relatively unstable environment.
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