期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 1, 页码 364-367出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201508576
关键词
biosynthesis; intermolecular transesterification; peptides; salinamide; thioesterase
资金
- NIH [R01-GM085770, R01-AI047818]
Salinamide A belongs to a rare class of bicyclic depsipeptide antibiotics in which the installation of a (4-methylhexa-2,4-dienoyl) glycine handle across a hexadepsipeptide core contributes to its chemical complexity and biological properties. Herein, we report the genetic and biochemical basis for salinamide construction in the marine bacterium Streptomyces sp. CNB-091, which involves a novel intermolecular transesterification reaction catalyzed by a type I thioesterase. Heterologous expression studies revealed the central role of the nonribosomal peptide synthetase Sln9 in constructing and installing the distinctive acylglycine basket handle of salinamide. Biochemical characterization of the Sln9 thioesterase domain established that transesterification of the serine residue of desmethylsalinamide E with acylated glycyl thioesters yields desmethylsalinamide C.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据