Highly selective two-photon fluorescent probe for imaging of nitric oxide in living cells

A new two-photon fluorescent probe, ADNO, for nitric oxide (NO) based on intramolecular photoinduced electron transfer (PET) mechanism displays a rapid response to NO with a remarkable fluorescent enhancement in PBS buffer. The excellent chemoselectivity of ADNO for NO over other ROS/RNS (reactive oxygen species or nitrogen species) and common metal ions was observed. Moreover, ADNO has been successfully applied in fluorescence imaging of NO of living cells using both one-photon microscopy (OPM) and two-photon microscopy (TPM). (C) 2013 Guo-Ping Li. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.