期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 50, 页码 15069-15073出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201507327
关键词
biomineralization; diatoms; DNP; hybrid materials; solid-state NMR
资金
- COST (Action) [TD1103]
- Deutsche Forschungsgemeinschaft [FOR 2038, BR 1278/24-1]
- NWO [722.012.002, 700.11.344, 700.26.121]
Diatom biosilica is an inorganic/organic hybrid with interesting properties. The molecular architecture of the organic material at the atomic and nanometer scale has so far remained unknown, in particular for intact biosilica. A DNP-supported ssNMR approach assisted by microscopy, MS, and MD simulations was applied to study the structural organization of intact biosilica. For the first time, the secondary structure elements of tightly biosilica-associated native proteins in diatom biosilica were characterized in situ. Our data suggest that these proteins are rich in a limited set of amino acids and adopt a mixture of random-coil and beta-strand conformations. Furthermore, biosilica-associated long-chain polyamines and carbohydrates were characterized, thereby leading to a model for the supramolecular organization of intact biosilica.
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