期刊
CHINESE CHEMICAL LETTERS
卷 22, 期 4, 页码 493-496出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2010.10.050
关键词
Molecular imprinting; Surface grafting; Catalytic hydrolysis; Paraoxon
资金
- National Natural Science Foundation of China [50978138]
Firstly, we synthesized N-methacryloyl-histidine monomer and N-methacryloyl-histidine-Cu2+ complex (MAH-Cu2+). Then the molecular imprinting polymers (MIP) has been prepared by surface grafting on uniform polystyrene (PS) core using reversible addition-fragmentation transfer polymerization (RAFT) with MAH-Cu2+ as the functional monomer, methyl paraoxon as the template to simulate phosphotriesterase (PTE). Finally, we have investigated the catalytic hydrolytic activities of MIP and non-imprinting polymers (NIP) to the template methyl paraoxon and the template analogue ethyl paraoxon respectively by UV spectrophotometry. The results showed that the catalytic hydrolytic activity of MIP to the template methyl paraoxon was highest and the value of k is 8.67 x 10(-5) mmol L-1 min(-1), 3.89-fold higher than MIP to the template analogue ethyl paraoxon, 2.79-fold higher than NIP to the template methyl paraoxon. The K-M, r(m) of MIP are also determined, and K-M = 3.95 x 10(-4) mol/L, r(m) = 2.12 mu mol/min. The MIP can be reused with only lose 7% of catalytic activity for four cycles. (C) 2010 Tian Ying Guo. Published by Elsevier B.A. on behalf of Chinese Chemical Society. All rights reserved.
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