期刊
CHINESE CHEMICAL LETTERS
卷 20, 期 3, 页码 358-361出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2008.10.025
关键词
Hemoglobin; ZnO; Multi-walled carbon nanotubes; Nafion; Bioelectrochemistry
Multi-walled carbon nanotubes (MWCNTs) were coated with ZnO by a hydrothermal method. The resulting nanocomposites were mixed with the Nafion solution to form a composite matrix for the fabrication of hemoglobin (Hb) biosensor. To prevent the leak of Hb molecules of the biosensor, silica sol-gel film was coated on the surface of the Hb/ZnO-MWCNTs/Nafion electrode. The silica sol-gel/Hb/ZnO-MWCNTs/Nafion film exhibited a pair of well-defined, quasi-reversible redox peaks. This biosensor showed excellent electrocatalytic activity to H2O2. The sensitivity and apparent Michaelis-Menten constant of this Hb biosensor to H2O2 were 1.31 A/(M cm(2)) and 82.8 mu mol/L, respectively, which indicated that Hb had high affinity to H2O2. (C) 2008 Dan Bi Tian. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.
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