Two new coumarin glucosides biosynthesized by transgenic hairy roots of Polygonum multiflorum

The glycosylation of hydroxylcoumarin was investigated by using suspension cultures of hairy roots of Polygonum multiflorum. Two new coumarin glucosides (3 and 4) were biosysthesized by regioselectively glycosylation of corresponding substrates (1 and 2) in the system. The structures of two products were identified as 7-hydroxy-4-methylcoumarin 5-O-beta-D-glucopyranoside and 7-hydroxy-3,4-dimethylcoumarin 5-O-beta-D-glucopyranoside on the ground of chemical and spectroscopic methods, respectively. (C) 2007 Rong Min Yu. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.