期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 54, 期 40, 页码 11716-11719出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201505348
关键词
circular dichroism; DNA structures; folding; G-quadruplexes; NMR spectroscopy
资金
- Slovenian Research Agency (ARRS) [P1-242, J1-6733]
Sequence inversion in G-rich DNA from 5'-> 3' to 3'-> 5' exerts a substantial effect on the number of structures formed, while the type of G-quadruplex fold is in fact determined by the presence of K+ or Na+ ions. The melting temperatures of G-quadruplexes adopted by oligonucleotides with sequences in the 5'-> 3' direction are higher than those of their 3'-> 5' counterparts with both KCl and NaCl. CD, UV, and NMR spectroscopy demonstrates the importance of primary sequence for the structural diversity of G-quadruplexes. The changes introduced by mere sequence reversal of the G-rich DNA segment have a substantial impact on the polymorphic nature of the resulting G-quadruplexes and their potential physiological roles. The insights resulting from this study should enable extension of the empirical rules for the prediction of G-quadruplex topology.
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