期刊
CHEMPHYSCHEM
卷 20, 期 2, 页码 318-325出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.201800652
关键词
Cancer; hyperpolarization; metabolism; NMR spectroscopy; parahydrogen
资金
- Airc (Italian Association for Cancer Research, TRIDEO call 2015)
- Compagnia di San Paolo (Athenaeum Research 2016) [CSTO164550]
The kinetics of metabolic processes can be assessed, in real time by means of MR hyperpolarized (HP) metabolites. [1-C-13]pyruvate, hyperpolarized by means of d-DNP, is, by far, the substrate most widely applied to the investigation of several pathologies characterized by deregulated glycolytic metabolic networks, including cancer. Hyperpolarization of [1-C-13]pyruvate by means of the cost effective, fast and easy to handle PHIP-SAH (para-hydrogen induced polarization-side arm hydrogenation) method opens-up a pathway for the application of HP metabolites to a wide range of cancer-related studies. Herein, we report the first application of PHIP-SAH hyperpolarized [1-C-13]pyruvate in the investigation of upregulated glycolysis in two murine breast cancer cell lines (168FARN and 4T1). The results obtained using HP pyruvate have been validated with a conventional biochemical assay and are coherent with previously-reported lactate dehydrogenase activity measured in those cells.
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