期刊
CHEMPHYSCHEM
卷 10, 期 9-10, 页码 1630-1641出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.200800856
关键词
amino acids; density functional calculations; gas phase; IRMPD spectroscopy; mass spectrometry
资金
- European Commission [15637]
- Fundacao para a Ciencia a Tecnologia [SFRH/BPD/22482/2005]
- Ecole Polytechnique
- European Union [15637]
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/22482/2005] Funding Source: FCT
Gas-phase infrared spectra of deprotonated phosphorylated amino acids ([pAA-H](-))-phosphoserine ([pSer-H](-)), phosphothreonine ([pThr-H](-)), and phosphotyrosine ([pTyr-H](-))-and of the dihydrogen phosphate anion H2PO4- have been recorded in the mid-IR region (650-2000 cm(-1)) under tandem mass spectrometry conditions. The experimental setup involved a Paul ion trap equipped with an electrospray ionization source coupled with a tunable free electron laser (FEL). Spectral assignment of the observed IRMPD bands and identification of the vibrational signatures of the phosphorylation have been performed by comparison with DFT calculations. The H2PO4- anion has been used as a simple model of a free deprotonated phosphate group, helping the identification of the IR signatures of phosphorylation. Our results show that deprotonation occurs on the phosphate group for the three amino acids. A comparison between the deprotonated and protonated phosphorylated amino acids is reported for the most important vibrational features.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据