期刊
CHEMPHYSCHEM
卷 9, 期 6, 页码 867-872出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cphc.200700591
关键词
DNA; enzymes; nanoparticles; Raman spectroscopy; surface chemistry
The growing interest in DNA diagnostics is addressed today by microarrays with fluoresence detection, In our approach, we utilize spatially defined arrays of short oligonucleotides on a modified glass surface. Surface enhanced resonance Roman scattering (SERRS) is used to obtain molecularly specific spectra of the Roman-active dye-labeled DNA. Nanoporticles produced by enzymatic silver deposition are used as SERS-active substrate. They grow directly on the modified oligonucleotides and only in the spatially defined areas on the chip. Furthermore, they potentially offer several advantages for SERS detection. The nanoporticles are characterized and their ability for use as SERS- and SERRS-active substrate is estimated. Three different Raman-active dyes are investigated for their potential for involvement in sequence specific DNA analysis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据