期刊
CHEMOTHERAPY
卷 55, 期 1, 页码 6-10出版社
KARGER
DOI: 10.1159/000166383
关键词
Quercetin; p16INK4a, demethylation; Flavonoids
资金
- National Basic Research Program of China [2007CB5119005]
Background: Quercetin is a flavonoid found ubiquitously in nature. Studies in vitro and in vivo have suggested that quercetin may have a protective role against colon cancer. Methods: We selected the human colon cancer cell line RKO to investigate the effects of quercetin in vitro. RKO cells were treated with different concentrations of quercetin. Results: In comparison to the control, quercetin was able to inhibit the growth of RKO cells, as measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Untreated RKO cells demonstrated almost complete methylation of the p16INK4a gene. Hypermethylation of the p16INK4a gene was successfully reversed after 120 h of treatment with quercetin. Expression of the p16INK4a gene was restored in a concentration-dependent manner. Conclusion: All these data suggest that quercetin has antitumor properties, probably via demethylation of the p16INK4a gene promoter. Copyright (C) 2008 S. Karger AG, Basel
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