期刊
CHEMOSPHERE
卷 93, 期 7, 页码 1436-1441出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2013.07.076
关键词
Excitation-emission matrix (EEM); Extracellular polymeric substances (EPS); Metal binding; Parallel factor analysis (PARAFAC); Sludge; Zeta potential
资金
- Natural Science Foundation of China [51322802, 21377123]
- Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China
The interactions between metals (Ca2+ and Hg2+) and extracellular polymeric substances (EPS) extracted from the aerobic and anaerobic sludge in wastewater treatment reactors were investigated using a combination of zeta potential measurement and 3-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy with parallel factor (PARAFAC) analysis. Results show that Ca2+ had no substantial effects on the EEM fluorescence spectra of the EPS, but their zeta potentials increased with the increasing Ca2+ dosage. However, Hg2+ had a significant effect on the EEM fluorescence spectra of the EPS, while their zeta potentials seemed not to be affected by the dose of Hg2+. The interactions between Hg2+ and EPS were elucidated using the fluorescence quenching with PARAFAC analysis, while the interactions between Ca2+ and EPS were evaluated by the zeta potential technique. The binding constants for Hg2+ and EPS were two orders of magnitude higher than those for Ca2+ and EPS, suggesting that the binding mechanisms between Ca2+ and EPS were different from those between Hg2+ and EPS. The results might be useful for understanding the roles of EPS in bacterial self-protection against heavy metals and the aggregate formation mechanisms through ionic bridging interactions. (C) 2013 Elsevier Ltd. All rights reserved.
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